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chromaspro

ChromasPro - DNA测序和分析软件

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ChromasPro用于将序列读数组装成contigs  。有一个图形contig编纂器 ,显示对齐的色谱图  。它还能够进行通常的序列分析 ,如限度性酶图谱、盛开读码框搜索和BLAST提交  。

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ChromasPro合用于高达几兆的DNA序列组装项目 ,以及根基的序列编纂和分析  。它可能组装来自Sanger测序仪(如ABI)和454和Illumina下一代测序仪的数据 ,若是有8GB内存的话 ,至多可组装1,000,000条序列  。ChromasPro拥有以下特点:

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  • 以Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF和纯文本体式打开序列  。

  • 以.ab1、.scf、.fasta和.fastq体式保留序列  。

  • 使用图形界面组装沉叠的序列 ,天生共识 ,并凸起显示闪动其词的处所供编纂  。

  • 使用质量数据自动去除低质量的序列 ,以提高序列组装  。

  • 使用参考序列作为组装的支架  。

  • 天生限度性位点和片段图 ,并列出切割、无切割和片段  。

  • 在G+C框架图的援手下 ,绘造盛开阅读框架图 ,并一键翻译ORFs  。

  • 打印色谱图、限度性位点和片段图以及盛开阅读框架图  。

  • 通过NCBI网站进行核苷酸和蛋白质BLAST搜索  。

  • 通过与ClustalW对接进行多沉比对  。

  • 回转和补充序列和色谱图  。

  • 通过正确匹配或optimal分列搜索序列  。

  • 在编纂核苷酸序列时显示翻译  。

  • 进行反向翻译并绘造核苷酸退化图  。

  • 绘造蛋白质的亲水性和抗原性  。

  • 复造色谱图部门的图像 ,以便粘贴到文档或演示文稿中  。

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系统要求:

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与Windows 7 sP1 ,8 ,10和11

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【英文介绍】

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ChromasPro is for assembly of sequence reads into contigs, with a graphical contig editor which displays aligned chromatograms. It also peforms general sequence analysis such as restriction enzyme mapping, open-reading-frame searches, and BLAST submission.

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ChromasPro is suitable for DNA sequence assembly projects up to a few megabases, and basic sequence editing and analysis. It is able to assemble data from Sanger sequencers such as ABI, and 454 and Illumina next-generation sequencers, with up to 1,000,000 sequences if 8 Gb RAM is available. ChromasPro has the following features:

  • Open sequences in Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF and plain text formats.

  • Save sequences in .ab1, .scf, .fasta and .fastq formats.

  • Assemble overlapping sequences using a graphical interface, generate a consensus and highlight ambiguities for editing.

  • Use quality data to automatically remove low quality sequence for improved sequence assembly.

  • Use a reference sequence as a scaffold for assembly.

  • Generate restriction site and fragment maps, and list cutters, non-cutters and fragments.

  • Map open reading frames, aided by a G+C frame plot, and translate ORFs with one click.

  • Print chromatograms, restriction site and fragment maps, and open reading frame maps.

  • Perform nucleotide and protein BLAST searches through the NCBI web site.

  • Perform multiple alignments by interfacing with ClustalW.

  • Reverse & complement sequences and chromatograms.

  • Search for sequences by exact matching or optimal alignment.

  • Display translations when editing nucleotide sequences.

  • Perform reverse translations and plot nucleotide degeneracy.

  • Plot hydrophilicity and antigenicity of proteins.

  • Copy an image of a chromatogram section for pasting into documents or presentations.

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