注：特此通知，BIOSOFT将于2022年12月31日终场买卖
。

电子邮件地址info@biosoft.com将在该日期后的2年内持续有效，以便向软件的注册用户提供法式激活代码和少量技术支持
。

?

QuantiScan是一款凝胶扫描分析软件
。QuantiScan还能够助你分析，扫描多种多样的资料成分
Ｄ芄唤ǔ５陌旃枰亲晃澳艿南韵衩芏燃，用于分析凝胶，污点和薄层层析板等
。?????????????????????????

?

用于Windows的QuantiScan拥有复杂的光密度计的职能，它可能分析多种资料，聚丙烯酰胺和琼脂糖凝胶，放射自显影图，TLC板等
。该法式将直接从TWAIN兼容扫描仪以及大无数BMP，JPG或未压缩的TIF文件接受图像进行分析
。对于质量合理的泳路凝胶，分析是自动化的，可实现很高的数据通量
。使用Quantiscan的手动模式几何能够分析类型的图像
。

?

数字，图形和图像输出能够打印为汇报，也能够另存为文件或复造到剪贴板
Ｄ芄唤饷芏确治錾⒉纪嫉腦，Y坐标输出到选项卡式表格中，以便在演示图形软件（例如Biosoft的FigSys）中进行沉新画图
。有单个的？，用于分析不陆续的印迹，也能够通过自动或手动印迹检测进行操作
。

?

泳路分析模式

凝胶拥有复杂的泳路处置和分析职能
Ｄ芄皇侄唇矶鹊某德，也能够使用自动车路检测模式创建
；褂幸恢种澳苣芄凰亩鞍垂潭ň嗬肷柚贸德，以用于拥有预先配置的规定车路阵列的板
。泳路能够倾斜或弯曲，以允许出现凝胶缺点
Ｄ芄桓丛煊韭诽烨，并使用光标键将副本地移动到的地位，以进行比力
。泳路在其图像和光密度测定图上均能够表有编号
。自动布景减法有六个选项：Minimum value，内插 Minimum value，谷值，边缘，多项式和手动
。用户选择的图像区域能够显示为3-D密度图，能够将其打印，保留或复造到剪切板
。

?

界说车路后，将自动绘造其光密度散布图
。而后能够通过在概括上手动记峰的中心和边月反指定峰，也能够使用复杂的峰搜索例程自动定峰值
。在的情况下，能够执行高斯峰的自动拟合
。这种自动拟合使用l east squares? Minimizes 技术将峰的序列和多项式布景拟合到给定的概括
。当前所选车路的图像在车路光密度测定曲线下方水平搁置
。光标节造的垂直线穿过概括和车路图像，有助于用户查抄峰
Ｄ芄辉龀こ叨瘸德返耐枷窈团渲梦募，以便与个车路进行比力
Ｄ芄坏髡堑亩云敕绞，以援手进行的并排比力
。峰面积是使用Simpson规定通过积分推算的，若是使用了自动拟合选项，则也会自动推算拟合峰的面积
Ｄ芄辉龀こ叨瘸德返耐枷窈团渲梦募，以便与个车路进行比力
Ｄ芄坏髡堑亩云敕绞，以援手进行的并排比力
。

?

能够界说尺度蛋白质的泳路进行校准，以自动推算分子量
。也能够使用已知的的尺度溶液（例如测定试剂盒提供溶液）进行校准（输入肇始行的地位，其摩尔沉量成分和容积前沿的地位，即可自动推算出未知数）
Ｄ芄槐Ａ舫叨扔韭返牟问越邪寮浔攘
Ｄ芄坏汲鲂Ｗ记咄技捌淠夂系淖
。天生峰参数的汇报，地位，宽度
。

?

印迹分析模式

QuantiScan提供了一种单个的模式来分许离散印迹
。印Ｄ芄蛔远觳饣蚴侄缢
。他们的数量，地位，，面积和光密度体积会自动汇报
。有效于自动分析规定分列的印迹的？
。自动布景减法是选项
Ｄ芄换诔叨扔〖５墓饷芏忍寤行Ｗ，这能够将印迹模式用于测定
。

?

?

【英文介绍】

?

QuantiScan for Windows has similar functionality to sophisticated densitometers but at a fraction of the cost. It is able to analyze a wide variety of material including polyacrylamide and agarose gels, autoradiograms, TLC plates, etc. The program will accept images for analysis directly from any TWAIN compliant scanner as well as most BMP, JPG or uncompressed TIF files. For laned gels of reasonable quality, the analysis is highly automated, allowing extremely high throughput of data. Almost any type of image can be analyzed with the manual modes of Quantiscan. Images can be pre-processed in a variety of ways within QuantiScan including crop, flip, invert, scroll and zoom.

?

?

?

Numeric, graphical and image output can be printed as reports and can also be saved as files or copied to the clipboard. The X,Y coordinates of densitometric profiles can output into a tabbed table for re-plotting in presentation graphics software such as Biosoft's FigSys. There is a separate module for analysis of discrete blots which can also be operated with automatic or manual blot detection.

?

Lane Analysis Mode

There is sophisticated lane processing and analysis for gels. Lanes of any width can be created manually or by using the unique automatic lane detection mode. There is also a facility to automatically set lanes at regular intervals for use with plates that have a preconfigured regular lane array. Lanes can be skewed or curved to allow for gel imperfections. Lane boundaries can be copied and the copies moved precisely into position with the cursor keys, for exact comparisons. Each lane can be labelled with an unique number both on its image and its densitometric profile plot. There are six options for automatic background subtraction: Minimum, Interpolated minimum, Valley, Edge, Polynomial and Manual. User-selected areas of images can be displayed as 3-D density maps which can be printed, saved or copied to the clipboard.

?

Once a lane has been defined, its densitometric profile plot is automatically drawn. Peaks can then be specified on the profile either manually by marking their center and edges, or automatically using a sophisticated peak search routine. Where appropriate, automatic fitting of Gaussian peaks can be performed. This automatic fitting uses a least-squares minimization technique to fit a sequence of peaks and a polynomial background to the given profile. The image of the currently-selected lane is shown laid-out horizontally under the lane densitometry profile. A cursor-controlled vertical line going across the profile and the lane image aids user examination of the peaks. The image and profile of a standard lane can be added for comparison with the first. Their alignment can be adjusted to aid accurate side-by-side comparisons. Peak areas are calculated by integration using Simpson's rule and, if the automatic fitting option has been used, the area of the fitted peak is also automatically calculated.

?

Calibration to a lane containing standard proteins may be made for automatic calculation of molecular weights. Calibration can also be made with a known standard solution such as those supplied with assay kits (just enter the position of its start line, its mol. wt. factor and the position of the solvent front, to give automatic calculation of mol. wts of unknowns). Parameters of any standard lane can be saved for between-plate comparisons. Calibration curve graphs and their fitted coordinates can be exported. Reports are generated for all peak parameters, including position, height, width, background and net area of peaks and, together with the graphical results, these can be printed, saved to disk or copied to the clipboard for transfer to other programs.

?

Blot Analysis Mode

QuantiScan offers a separate mode to analyze discrete blots. The blots can be automatically detected or manually defined. Their number, position, height, area and densitometric volume are automatically reported. There is a special module for automatic analysis of regularly arrayed blots. Automatic background subtraction is an option. Calibration, based on densitometric volume of standard blots can be done and this allows blot mode to be used for assays.

?